Comparison of Bone Marrow-derived Mononuclear Cells vs. Mesenchymal Stem Cells in Protecting Random-pattern Skin Flap Survival in Rats

Both cultured Bone Marrow Mesenchymal Stem Cells (BMSCs) and non-cultured Bone Marrow Mononuclear Cells (BM-MNCs) can improve random-pattern skin flap survival. However, whether prior culture expansion of bone marrow cells is beneficial for this therapy remains unclear. In the current study, the protective effects of BM-MNCs and BMSCs derived from identical bone marrow aspirates were compared in a random-pattern skin flap rat model. The mean skin flap survival rates were 71.6 ± 8.4% in the BM-MNC-treated group and 66.2 ± 3.1% in the BMSCtreated group, both of which were significantly higher than the control group (55.9 ± 3.4%). The protective effects were confirmed by blood perfusion analysis and vessel density assay. However, no significant difference was observed between the cell transplanted groups. These results indicate that the current method for pre-culture of BMSCs does not bring therapeutic benefits in skin flap protection. Therefore, BM-MNCs without pre-culture might be more practicable in the clinical setting. Comparison of Bone Marrow-derived Mononuclear Cells vs. Mesenchymal Stem Cells in Protecting Random-pattern Skin Flap Survival in Rats Peng Xu, Yang Lu, Zhenxing Wang, Jie Lian, Guangdong Zhou, Wei Liu, Yilin Cao, Wei Li* and Wen Jie Zhang* Department of Plastic and Reconstructive Surgery, National Tissue Engineering Center of China, Shanghai 200011, China *Corresponding authors: Wei Li, Department of Plastic and Reconstructive Surgery, Shanghai 9th People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Tissue Engineering, National Tissue Engineering Center of China, Shanghai 200011, China, Tel: +86-21-23271699; Fax: +86-21-53078128; E-mail: [email protected] Wen Jie Zhang, Department of Plastic and Reconstructive Surgery, Shanghai 9th People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Tissue Engineering, National Tissue Engineering Center of China, Shanghai 200011, China, Tel: +86-21-23271699; Fax: +86-21-53078128; E-mail: [email protected] Received July 09, 2016; Accepted August 16, 2016; Published August 22, 2016 Citation: Xu P, Lu Y, Wang Z, Lian J, Zhou G, et al. (2016) Comparison of Bone Marrow-derived Mononuclear Cells vs. Mesenchymal Stem Cells in Protecting Random-pattern Skin Flap Survival in Rats. J Stem Cell Res Ther 6: 355. doi: 10.4172/2157-7633.1000355 Copyright: © 2016 Xu P, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.